Review



aflii restriction enzyme  (New England Biolabs)


Bioz Verified Symbol New England Biolabs is a verified supplier
Bioz Manufacturer Symbol New England Biolabs manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 97

    Structured Review

    New England Biolabs aflii restriction enzyme
    Aflii Restriction Enzyme, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 569 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/aflii+restriction+enzymes/AflII/pmc13127484-23-0-4
    Average 97 stars, based on 569 article reviews
    aflii restriction enzyme - by Bioz Stars, 2026-10
    97/100 stars

    Images

    Related Articles

    Purification:

    Article Title: Novel GUCY2C variant causing familial diarrhea in a Mennonite kindred and a potential therapeutic approach.
    Article Snippet: 2.4 | Cloning and construction of GUCY2C control and c.2381A > T variant IRES GFP plasmids Expression vectors pcDNA3.1(+) containing full GUCY2C cDNA sequence with and without the c.2381A > T variant were constructed by BioMatik (BioMatik, Ontario, Canada). .. Vectors were treated with NotI and AflII restriction enzymes (New England BioLabs, Ipswich, MA) and inserts were gel purified using the Zymoclean Gel DNA recovery kit (ZymoResearch, Irvine, CA), then ligated into the multicloning site of a pcDNA3.1(+) IRES-GFP expression vector (Addgene, Watertown, MA). .. The ligation product was introduced into XL-1 Blue supercompetent cells (Agilent, Santa Clara, CA), and Sanger sequencing was used to confirm the full GUCY2C insert sequence (Supplementary Table S1).

    Expressing:

    Article Title: Novel GUCY2C variant causing familial diarrhea in a Mennonite kindred and a potential therapeutic approach.
    Article Snippet: 2.4 | Cloning and construction of GUCY2C control and c.2381A > T variant IRES GFP plasmids Expression vectors pcDNA3.1(+) containing full GUCY2C cDNA sequence with and without the c.2381A > T variant were constructed by BioMatik (BioMatik, Ontario, Canada). .. Vectors were treated with NotI and AflII restriction enzymes (New England BioLabs, Ipswich, MA) and inserts were gel purified using the Zymoclean Gel DNA recovery kit (ZymoResearch, Irvine, CA), then ligated into the multicloning site of a pcDNA3.1(+) IRES-GFP expression vector (Addgene, Watertown, MA). .. The ligation product was introduced into XL-1 Blue supercompetent cells (Agilent, Santa Clara, CA), and Sanger sequencing was used to confirm the full GUCY2C insert sequence (Supplementary Table S1).

    Staining:

    Article Title: Complete genome sequence and transcriptomics analyses reveal pigment biosynthesis and regulatory mechanisms in an industrial strain, Monascus purpureus YY-1
    Article Snippet: .. High-molecular-weight DNA was then immobilized as individual molecules onto Optical Chips, digested with AflII restriction enzymes (New England Biolabs, USA), fluorescently stained with a staining kit (OpGen) and positioned onto an automated fluorescent microscope system for image capture and fragment size measurement; these steps resulted in high-resolution single-molecule restriction maps. ..

    Article Title: Optical mapping as a routine tool for bacterial genome sequence finishing
    Article Snippet: .. The high molecular weight DNA was then immobilized as individual molecules onto Optical Chips, digested with EagI or AflII restriction enzymes (New England Biolabs), fluorescently stained with YOYO-1 (Invitrogen) and positioned onto an automated fluorescent microscope system for image capture and fragment size measurement, resulting in high resolution single-molecule restriction maps. ..

    Microscopy:

    Article Title: Complete genome sequence and transcriptomics analyses reveal pigment biosynthesis and regulatory mechanisms in an industrial strain, Monascus purpureus YY-1
    Article Snippet: .. High-molecular-weight DNA was then immobilized as individual molecules onto Optical Chips, digested with AflII restriction enzymes (New England Biolabs, USA), fluorescently stained with a staining kit (OpGen) and positioned onto an automated fluorescent microscope system for image capture and fragment size measurement; these steps resulted in high-resolution single-molecule restriction maps. ..

    Article Title: Optical mapping as a routine tool for bacterial genome sequence finishing
    Article Snippet: .. The high molecular weight DNA was then immobilized as individual molecules onto Optical Chips, digested with EagI or AflII restriction enzymes (New England Biolabs), fluorescently stained with YOYO-1 (Invitrogen) and positioned onto an automated fluorescent microscope system for image capture and fragment size measurement, resulting in high resolution single-molecule restriction maps. ..

    Clone Assay:

    Article Title: The basic domain of Suv39h2 buffers mitoxantrone-induced heterochromatin destabilization
    Article Snippet: .. Gene fragments were flanked by XhoI and AflII restriction sites (IDT) and cloned into the backbone using XhoI and AflII restriction enzymes (NEB), as previously described in Velazquez et al. All plasmid sequences were confirmed by Sanger sequencing. .. To generate D5-Suv39h1-EGFP, D5-Suv39h2-EGFP, D5-Suv39h2ΔBD-EGFP, and D5-BD-Suv39h1-EGFP cell lines, the plasmids pCAGGS-Suv39h1-EGFP-IRES-Puro, pCAGGS-Suv39h2-EGFP-IRES-Puro, pCAGGS-Suv39h2ΔBD-EGFP-IRES-Puro, and pCAGGS-BD-Suv39h1-EGFP-IRES-Puro were first linearized using the PvuI restriction enzyme (NEB).

    Article Title: The basic domain of Suv39h2 buffers mitoxantrone-induced heterochromatin destabilization.
    Article Snippet: .. Gene fragments were flanked by XhoI and AflII restriction sites (IDT) and cloned into the backbone using XhoI and AflII restriction enzymes (NEB), as previously described in Velazquez et al.8 All plasmid sequences were confirmed by Sanger sequencing. .. Generation of MEF cells expressing EGFP-tagged Suv39h enzymes To generate D5-Suv39h1-EGFP, D5-Suv39h2-EGFP, D5-Suv39h2ΔBD-EGFP, and D5-BD-Suv39h1-EGFP cell lines, the plasmids pCAGGS-Suv39h1-EGFP-IRES-Puro, pCAGGS-Suv39h2-EGFP-IRES-Puro, pCAGGS-Suv39h2ΔBD-EGFP-IRES-Puro, and pCAG GS-BD-Suv39h1-EGFP-IRES-Puro were first linearized using the PvuI restriction enzyme (NEB).

    Plasmid Preparation:

    Article Title: The basic domain of Suv39h2 buffers mitoxantrone-induced heterochromatin destabilization
    Article Snippet: .. Gene fragments were flanked by XhoI and AflII restriction sites (IDT) and cloned into the backbone using XhoI and AflII restriction enzymes (NEB), as previously described in Velazquez et al. All plasmid sequences were confirmed by Sanger sequencing. .. To generate D5-Suv39h1-EGFP, D5-Suv39h2-EGFP, D5-Suv39h2ΔBD-EGFP, and D5-BD-Suv39h1-EGFP cell lines, the plasmids pCAGGS-Suv39h1-EGFP-IRES-Puro, pCAGGS-Suv39h2-EGFP-IRES-Puro, pCAGGS-Suv39h2ΔBD-EGFP-IRES-Puro, and pCAGGS-BD-Suv39h1-EGFP-IRES-Puro were first linearized using the PvuI restriction enzyme (NEB).

    Article Title: Broad-Host-Range Genetic Tools for Cyanobacterial Engineering
    Article Snippet: OD750 readings for cell density were measured daily using the Tecan Infinite® M200 plate reader (TECAN). .. 61 Section III Plasmid Construction To construct pAM5684, pAM5409 was digested using SbfI and AflII restriction enzymes (New England BioLabs) to remove the yfp reporter gene immediately downstream of the conII promoter. ..

    Article Title: A living material platform for the biomineralization of biosilica
    Article Snippet: .. The PCR product and the template plasmid were digested with BamHI and AflII restriction enzymes (NEB) and then the digested fragments were ligated by T4 ligase (NEB). ..

    Article Title: The basic domain of Suv39h2 buffers mitoxantrone-induced heterochromatin destabilization.
    Article Snippet: .. Gene fragments were flanked by XhoI and AflII restriction sites (IDT) and cloned into the backbone using XhoI and AflII restriction enzymes (NEB), as previously described in Velazquez et al.8 All plasmid sequences were confirmed by Sanger sequencing. .. Generation of MEF cells expressing EGFP-tagged Suv39h enzymes To generate D5-Suv39h1-EGFP, D5-Suv39h2-EGFP, D5-Suv39h2ΔBD-EGFP, and D5-BD-Suv39h1-EGFP cell lines, the plasmids pCAGGS-Suv39h1-EGFP-IRES-Puro, pCAGGS-Suv39h2-EGFP-IRES-Puro, pCAGGS-Suv39h2ΔBD-EGFP-IRES-Puro, and pCAG GS-BD-Suv39h1-EGFP-IRES-Puro were first linearized using the PvuI restriction enzyme (NEB).

    Sequencing:

    Article Title: The basic domain of Suv39h2 buffers mitoxantrone-induced heterochromatin destabilization
    Article Snippet: .. Gene fragments were flanked by XhoI and AflII restriction sites (IDT) and cloned into the backbone using XhoI and AflII restriction enzymes (NEB), as previously described in Velazquez et al. All plasmid sequences were confirmed by Sanger sequencing. .. To generate D5-Suv39h1-EGFP, D5-Suv39h2-EGFP, D5-Suv39h2ΔBD-EGFP, and D5-BD-Suv39h1-EGFP cell lines, the plasmids pCAGGS-Suv39h1-EGFP-IRES-Puro, pCAGGS-Suv39h2-EGFP-IRES-Puro, pCAGGS-Suv39h2ΔBD-EGFP-IRES-Puro, and pCAGGS-BD-Suv39h1-EGFP-IRES-Puro were first linearized using the PvuI restriction enzyme (NEB).

    Article Title: The basic domain of Suv39h2 buffers mitoxantrone-induced heterochromatin destabilization.
    Article Snippet: .. Gene fragments were flanked by XhoI and AflII restriction sites (IDT) and cloned into the backbone using XhoI and AflII restriction enzymes (NEB), as previously described in Velazquez et al.8 All plasmid sequences were confirmed by Sanger sequencing. .. Generation of MEF cells expressing EGFP-tagged Suv39h enzymes To generate D5-Suv39h1-EGFP, D5-Suv39h2-EGFP, D5-Suv39h2ΔBD-EGFP, and D5-BD-Suv39h1-EGFP cell lines, the plasmids pCAGGS-Suv39h1-EGFP-IRES-Puro, pCAGGS-Suv39h2-EGFP-IRES-Puro, pCAGGS-Suv39h2ΔBD-EGFP-IRES-Puro, and pCAG GS-BD-Suv39h1-EGFP-IRES-Puro were first linearized using the PvuI restriction enzyme (NEB).

    Polymerase Chain Reaction:

    Article Title: Expression of prophage-encoded endolysins contributes to autolysis of Lactococcus lactis.
    Article Snippet: .. PCR fragment pi305 was digested with BsaI, ApaI and AflII restriction enzymes (New England Biolabs, Ipswich, MA, USA) to generate cloning overhangs and digest co-amplified pi149 and pi252 PCR fragments, respectively. ..

    Article Title: A living material platform for the biomineralization of biosilica
    Article Snippet: .. The PCR product and the template plasmid were digested with BamHI and AflII restriction enzymes (NEB) and then the digested fragments were ligated by T4 ligase (NEB). ..

    Cloning:

    Article Title: Expression of prophage-encoded endolysins contributes to autolysis of Lactococcus lactis.
    Article Snippet: .. PCR fragment pi305 was digested with BsaI, ApaI and AflII restriction enzymes (New England Biolabs, Ipswich, MA, USA) to generate cloning overhangs and digest co-amplified pi149 and pi252 PCR fragments, respectively. ..

    High Molecular Weight:

    Article Title: Optical mapping as a routine tool for bacterial genome sequence finishing
    Article Snippet: .. The high molecular weight DNA was then immobilized as individual molecules onto Optical Chips, digested with EagI or AflII restriction enzymes (New England Biolabs), fluorescently stained with YOYO-1 (Invitrogen) and positioned onto an automated fluorescent microscope system for image capture and fragment size measurement, resulting in high resolution single-molecule restriction maps. ..

    Construct:

    Article Title: Broad-Host-Range Genetic Tools for Cyanobacterial Engineering
    Article Snippet: OD750 readings for cell density were measured daily using the Tecan Infinite® M200 plate reader (TECAN). .. 61 Section III Plasmid Construction To construct pAM5684, pAM5409 was digested using SbfI and AflII restriction enzymes (New England BioLabs) to remove the yfp reporter gene immediately downstream of the conII promoter. ..



    Similar Products

    97
    New England Biolabs aflii restriction enzyme
    Aflii Restriction Enzyme, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/aflii+restriction+enzymes/AflII/pmc13127484-23-0-4
    Average 97 stars, based on 1 article reviews
    aflii restriction enzyme - by Bioz Stars, 2026-10
    97/100 stars
      Buy from Supplier

    97
    New England Biolabs aflii restriction enzymes
    Aflii Restriction Enzymes, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/aflii+restriction+enzymes/AflII/pmc13127484-383-19-22
    Average 97 stars, based on 1 article reviews
    aflii restriction enzymes - by Bioz Stars, 2026-10
    97/100 stars
      Buy from Supplier

    99
    New England Biolabs r0146s aflii restriction enzyme new england biolabs
    R0146s Aflii Restriction Enzyme New England Biolabs, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/aflii+restriction+enzymes/XhoI/pm42063540-214-140-144
    Average 99 stars, based on 1 article reviews
    r0146s aflii restriction enzyme new england biolabs - by Bioz Stars, 2026-10
    99/100 stars
      Buy from Supplier

    97
    New England Biolabs restriction enzymes aflii
    Restriction Enzymes Aflii, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/aflii+restriction+enzymes/AflII/bio_rxiv__2025__05__09__653172-388-11-16
    Average 97 stars, based on 1 article reviews
    restriction enzymes aflii - by Bioz Stars, 2026-10
    97/100 stars
      Buy from Supplier

    97
    New England Biolabs restriction enzyme aflii
    Restriction Enzyme Aflii, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/aflii+restriction+enzymes/AflII/pmc12111687-202-9-27
    Average 97 stars, based on 1 article reviews
    restriction enzyme aflii - by Bioz Stars, 2026-10
    97/100 stars
      Buy from Supplier

    Image Search Results